<i>OsTPP1</i> regulates seed germination through the crosstalk with abscisic acid in rice

Seed germination is essential for direct seeding in rice. It has been demonstrated that trehalose-6-phosphate phosphatase 1 (OsTPP1) plays roles improving yield and stress tolerance In this study, the of OsTPP1 on seed rice were investigated. The tpp1 mutant germinated slower than wild-type (WT), which can be restored by exogenous trehalose. seeds showed higher ABA content compared with WT seeds. was hypersensitive to catabolism inhibitor (Dinicozanole). Furthermore, two genes downregulated responsible increased concentrations, trehalose transcripts genes, suggesting acted same signaling pathway. Further analysis a transcription factor OsGAMYB an activator OsTPP1, expression decreased both endogenous ABA, subsequently reducing suggested new pathway required addition, ABA-responsive especially OsABI5, invoved OsTPP1-mediated germination. Overall, our study provided pathways controlled through crosstalk